Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells
نویسندگان
چکیده
منابع مشابه
Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...
متن کاملoptimization of human peripheral blood mononuclear cells (pbmcs) cryopreservation
cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...
متن کاملOptimization of Human Peripheral Blood Mononuclear Cells (PBMCs) Cryopreservation
Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...
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mitochondria play key roles in many cell functions including energy production, fatty acid metabolism, pyrimidine biosynthesis, calcium homeostasis, and aging. they also regulate crucial signaling cascades such as apoptosis and oxidative stress. the proteome is often used to investigate the functional correlations on protein levels. based upon the human, genome there is estimated 2000 to 2500 a...
متن کاملThe Influence of Iron Loading and Iron Chelation on the Proliferation and Telomerase Activity of Human Peripheral Blood Mononuclear Cells
Background: Iron is an essential trace element in cell proliferation. Several investigations demonstrate that iron deprivation inhibits cell proliferation. However, the impact of iron on telomerase activity of activated lymphocytes remains unexplained to date. Objective: In this study, the effect of iron on the proliferation and telomerase activity of lymphocytes stimulated by phytohemagglutini...
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ژورنال
عنوان ژورنال: Current Protocols in Toxicology
سال: 2017
ISSN: 1934-9254,1934-9262
DOI: 10.1002/cptx.31